Figure 5.

Complementation between B″α K251D and Aα D61K. Wildtype (WT) or mutant B″α-GFP was coexpressed with WT or mutant Aα-EE at 3:1 plasmid ratios, and PP2A holoenzyme assembly was assessed by Aα-EE immunoprecipitation (IP) and immunoblotting for the indicated PP2A subunits. A, Western blottings representative of four independent transfections. Positions of molecular mass markers (in kDa) are indicated on the right. B–E, quantification of transfected B″α-GFP (B), endogenous pan-B (C), B′δ (D), and B′ϵ (E) in the immunoprecipitate. Signals are normalized to immunoprecipitated Aα-EE levels in the same lane and to the WT/WT condition (means ± S.E. and individual data points from four transfections). Significance levels are indicated as p values determined by one-way ANOVA followed by Dunnett's post hoc test.