Table 3.

Primers used in this study

Primer designation or category	Primer sequence
Primer pairs used for generating the underlined mutations in cps1B
D133A/D135A
CL162	5′-TTACCTTTATTGCGCCAGCGGATTTTCTTAG-3′
CL163	5′-CCCATTCTGTTTGTACGTATTTTAGTCC-3′
H587A
IB06	5′-ATTTTTACAGGCGGGTATAACTAAAGATGATTTATCTCAATGGTTCAATAC-3′
IB07	5′-GTTATACCCGCCTGTAAAAATATAAATTTTTTACTAAAATCATAATTGTCGCC-3′
H717A
IB08	5′-AATATTAGGCGCTGGTGCAAAAATAACTTTATATCCTAAATTTTTAATT-3′
IB09	5′-TTGCACCAGCGCCTAATATTGAACCATATTTAAATGAGTTTAACATCCCCC-3′
Primers for the amplification of cps1D
IB30	5′-GGTGGTGGTGGTGGTGCTCGAGCTCTGTTTCCATAGTATTTTTACAG-3′
IB31	5′-CGAGCACTTCACCAACAAGGACCATAGCATATGAATATTAAAGAAATATATAAAAAGATAAAGAATG-3′
Primer pairs used for generating the underlined mutations in cps1D
H242A
IB32	5′-ATGATGCTGCTCCAATTTACGATGTAAACACAGGC-3′
IB33	5′-AATTGGAGCAGCATCATCAGTACGAATTTGATTATTTGTTG-3′
W265A
IB34	5′-ACCATGTTGCGATTGGATATGGAGCAACAATTTTATCTGG-3′
IB35	5′-ATCCAATCGCAACATGGTCGCCTATTTGAATATCTTTAG-3′