Figure 7.
ZNT5 recruits ZNT6 to Golgi apparatus. A, ZNT6 expression was similar in WT, HAP–Z5–KO, HAP–Z5Z7–DKO, and HAP–Z5Z7–DKO cells stably expressing WT ZNT5 or ZNT5H451A. Endogenous ZNT6 was detected with anti-ZNT6 antibody. CNX, loading control. B–D, ZNT6 localization to the Golgi apparatus depends on the expression of ZNT5 but not ZNT7. ZNT6 was co-stained with GM130 (B), ZNT5 (C), or ZNT7 (D) in the indicated cells. Merged images with DAPI staining are also shown. Each experiment was performed at least three times, and representative results from independent experiments are shown.