Figure 4.

DEGs in fatty acid transport, trafficking and oxidation, lipid droplet biogenesis, and peroxisome biogenesis correlate with increased size of lipid droplets in the Fatp2^−/− liver. A, increased expression of genes (red) encoding proteins involved in fatty acid transport, trafficking, β-oxidation (mitochondrial (left) and peroxisomal (right)), and peroxisome biogenesis pathways. B, DEGs enriched in the pathways leading to triglyceride and lipid droplet biogenesis. For A and B, DEGs increased in expression are colored in red; for genes noted with superscript m or f, expression changed only in male or female mice, respectively; genes noted in black were detected but were unchanged between the control and Fatp2^−/− mice. C, control (left) and Fatp2^−/− (right) liver sections stained with Oil Red O; images are representative of n = 4 mice. D, quantification of Oil Red O staining (left, indicative of lipid droplets) as noted in Fig. 4C using ImageJ software; data are ± S.D. (error bars) (n = 4). Quantification of neutral lipids (right) as fatty acid methyl esters from control (gray) and Fatp2^−/− (black) liver (μg of FA/mg of tissue ± S.D. (n = 4); *, p ≤ 0.05 from Student's t test.