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. 2020 Mar 12;295(17):5626–5639. doi: 10.1074/jbc.RA119.011556

Figure 1.

Figure 1.

Expression of pncRNA-D is enhanced after osmotic stress, and TLS is essential for CCND1 repression by pncRNA-D. A, schematic drawing of CCND1 upstream region (top) and relative expression level of pncRNA-D in HeLa cell after indicated stimuli was detected by RT-qPCR (bottom). Expression levels in control cells were set to a value of 1.0 (n = 3). Black boxes in the schematic drawing indicate the regions examined in Fig. 2B. B, relative expression level (normalized to GAPDH) of CCND1 and pncRNA-D was evaluated by RT-qPCR. Expression level of pncRNA-D in control cells was set to a value of 1.0 (n = 5). C, relative expression level (normalized to GAPDH) of pncRNA-D (left panel) and CCND1 (right panel) of WT and TLS-KO HAP1 cells before and after sorbitol treatment. For TLS-KO HAP-1 cells, GFP-tagged TLS was overexpressed at the same time with sorbitol treatment (light gray box). Expression levels in control cells were set to a value of 1.0 (n = 4). D, relative expression level (normalized to GAPDH) of pncRNA-D after 0.4 m sorbitol treatment with or without actinomycin D treatment. Expression levels in control cells were set to a value of 1.0 (n = 5). *, p < 0.05; **, p < 0.01.