Figure 6.

pncRNA-D rescues cell cycle arrest caused by decreased m⁶A modification level. A, HeLa cells were sorted into G₀/G₁, S, and G₂/M phase cells by FACS (top panel), and the expression level of CCND1 and pncRNA-D was measured in each fraction (bottom panel). The expression level of pncRNA-D in G₀/G₁ phase control cells was set to a value of 1.0 (n = 5). B, histograms of FACS after indicated treatments. Representative figures of three distinct experiments are shown by histograms. Overlaid graph of HeLa cells treated with si-pncRNAD in addition to si-METTL3, si-YTHDC1, or 0.4 m sorbitol treatment are shown in light blue histograms. C and D, population of each fraction of experiments in B were analyzed statistically for siRNA treated cells (C) or 0.4 m sorbitol treated cells (D). E, relative expression level of CCND1 after siRNA treatments. Expression level of in control cells was set to a value of 1.0 (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.005.