Skip to main content
. 2020 Mar 12;295(17):5626–5639. doi: 10.1074/jbc.RA119.011556

Table 1.

Primers used in the study

Sense Antisense
RT-qPCR
    pncRNA-D 5′-GGGACCCTCTCATGTAACCA-3′ 5′-GAGCCGGCATAATTCAGAAC-3′
    GAPDH 5′-CATCACCATCTTCCAGGAGCG-3′ 5′-CACCACCTTCTTGATGTCATA-3′
    CCND1 5′-CGTGGCCTCTAAGATGAAGG-3′ 5′-CTGGCATTTTGGAGAGGAAG-3′
    METTL3 5′-CCCTGTCGCAAGCTGCACTT-3′ 5′-AACTCCATGGCCCTGCCTGT-3′
    METTL14 5′-TTCGACCAGGCTGGCTCACA-3′ 5′-AGCCACCTCTGTGTGCTCCT-3′
    WTAP 5′-GCAGCTGCTCCATTGTGCCT-3′ 5′-TTCCCTGCGTGCAGACTCCT-3′
    FTO 5′-ACCGAGTGGCAGAGTGCTCA-3′ 5′-CGGGCAATTCGTGACTGGCA-3′
    ALKBH5 5′-TCGTCAACAGCGCCGTCATC-3′ 5′-AACACGGAGCTGCTCAGGGA-3′
ChIP
    −3 kb control 5′-CAGGGAATCTCTGGTGGATT-3′ 5′-GGCGATTCTAATTCCTCGAC-3′
    pncRNA-D promoter 5′-GAGGAATTCACCCTGAAAGGTG-3′ 5′-CAAACCACCGTACCCTCTTA-3′
    CCND1 promoter 5′-AACGTCACACGGACTACAG-3′ 5′-ACTCTGCTGCTCGCTGCTAC-3′
CRISPR guide RNA
    pncRNA-D 453–476 5′-CACCGAAATATTAGTCCCCTCTGCC-3′ 5′-AAACGGCAGAGGGGACTAATATTTC-3′
Luciferase assay
    pncRNA-D promoter 5′-ATGGTACCCCTCACGCTCACGAATT-3′ 5′-ACGAAGCTTGCTCCAGGACTTTGCA-3′
Overexpression
    GFP--TLS 5′-ACCGCTCGAGATGGCCTCAAACGATTATAC-3′ 5′-TATACTGCAGCTAATACGGCCTCTCCCTGC-3′
    Strep-YTHDC1 5′-AGAATTCAGCGGCTGACAGTCGGGA-3′ 5′-GCCCTGCAGTTATCTTCTATATCGA-3′