Figure 6.

Gαq3 isoform regulates rhodopsin synthesis. A, Western blots of Rh1 levels in each genotype. Rh1 quantification for each genotype is presented in the lower panel. B, Western blots of Rh1 levels in w¹¹¹⁸ and Gαq^221c mutant flies. Rh1 quantification for each genotype is presented in the right panel. C, Western blots of Rh1 levels in w¹¹¹⁸, nlr;GMR-GAL4, and nlr;GMR-GAL4/UAS::Gαq3 flies. Rh1 quantification for each genotype is presented in the right panel. D, Rh1 distribution in developing w¹¹¹⁸ (left) and nlr mutant retina (right). Fly heads were collected at 100 h after pupal formation and eclosion, and sections were prepared and stained with anti-Rh1 (green) and anti-INAD (red) antibodies. The Rh1 signal remaining in the cytoplasm is indicated with arrows. Scale bar, 5 μm. E, quantification of Rh1 remaining in the cytoplasm at 100 h after pupal formation for each genotype.