Fig. 5.
Flow cytometry analysis on whole fruit extract treatment on ZIKV placenta infection. HTR-8/SVneo cells were pre-treated with 100 μg mL−1 of chloroquine diphosphate or 10 μg mL-1 of whole fruit extract or peel extract for 1 h, and further infected with MR766 (A and D) or PE243 (B and D) ZIKV strains. Cellular gate for size and granularity of HTR-8/SVneo cells is depicted in C. In D, first line depicts MR766 strain infection and second line PE243 infection, both with negative controls (C-), ZIKV group, chloroquine diphosphate + ZIKV, whole fruit + ZIKV and peel + ZIKV. The treatments were able to prevent infection of both strains of ZIKV in trophoblast cells (A, B and D). Experiments were performed in n = 6.