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. 2020 Apr 27;11(4):296. doi: 10.1038/s41419-020-2492-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a PCR array in ZNF750 knockdown cells using the kit of Cancer Pathway Finder PCR Array. b, c qRT-PCR showed the expression of FOXC2 in ZNF750 knockdown cells (b) and ZNF750-wt cells (c). GAPDH was performed as a loading control. Statistical analysis was performed with a two-sided t test. d Representative images of tube formation (upper), migration (middle) and invasion(lower) of HUVEC. e The conditioned medium from FOXC2 knockdown in ZNF750 knockdown cells reversed the effect on tube formation (left), migration (middle) and invasion(right) of HUVEC. f Angiogenic biomarkers ANGPT2, FLT1, CCL2 and FGF2 were detected by qRT-PCR in ZNF750 knockdown cells and FOXC2 knockdown in ZNF750 knockdown cells. Statistical analysis was performed with one-way ANOVA. **p < 0.01, ***p < 0.0001.