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. 2020 Apr 27;11(4):294. doi: 10.1038/s41419-020-2475-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a qRT-PCR analysis of miR-548t-5p expression in 50 pairs of human PC tissues and adjacent non-neoplastic tissues. b As analyzed by qRT-PCR, miR-548t-5p expression in PC tissues was significantly lower than that in the corresponding adjacent non-neoplastic tissues. c Representative ISH images of miR-548t-5p expression. Scale bar, 100 μm in ×100, 50 μm in ×400. d A histogram showing the ISH results of miR-548t-5p expression in the 95 pairs of human PC tissues and adjacent non-neoplastic tissues. e MicroRNA FISH showed that miR-548t-5p was mostly located in the cytoplasm. Magnification, ×400; scale bar, 50 μm. f miR-548t-5p expression in normal human pancreatic ductal cell line hTERT‐HPNE and PC cell lines BXPC-3, CFPAC-1, COLO-357, MIAPACA-2, and PANC-1 by qRT-PCR. g Kaplan–Meier curves for overall survival (OS) by miR-548t-5p expression. The data are presented as mean ± SD from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.