Fig. 7.

ROS mediate the prorenin-induced activation of the NLRP3 inflammasome. A Representative images of cytoplasmic ROS production in control, prorenin, and prorenin with NAC groups using DCFH-DA-associated fluorescence assays. B ROS quantification indicated by fluorescent intensity in the different groups. C Immunoblots of the components of the NLRP3 inflammasome (NLRP3, ASC, and caspase-1), pro-IL-1β, and its mature product IL-1β in prorenin with or without the ROS scavenger NAC. D Statistics for experiments as in C. E Caspase-1 activity. Data represent the mean ± SEM; n = 6, *P < 0. 05 vs Ctrl; ^#P < 0. 05 vs prorenin, one-way ANOVA with unpaired t test; scale bar, 15 μm.