Fig. 8.

The increased NLRP3 inflammasome activation and NLRP3 translocation from cytoplasm to mitochondria are attenuated by co-treatment with prorenin and NAC. A Representative images of primary microglia co-stained with NLRP3 (red) and ACS (green). B Densitometric quantification of immunofluorescent staining for NLRP3 in different groups. C Co-localization of NLRP3 and ASC in control, prorenin, and prorenin plus NAC groups. D Representative images of primary microglia stained with NLRP3 (red) and mito-tracker (green). E Percentage co-localization of NLRP3 imunoreactivity with mitochondria. Data represent the mean ± SEM; n = 6; *P < 0.05 vs Ctrl; ^#P < 0.05 vs prorenin, one-way ANOVA with unpaired t test; scale bars, 15 μm in A and D.