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. 2020 Apr 21;11:223. doi: 10.3389/fendo.2020.00223

Figure 2.

Figure 2

The effects of eplerenone on the morphological characteristics were examined by histological analysis of the eWAT and liver of mice. Three groups of mice (normal diet-fed mice, ND: white bar; high-fat diet-fed mice, HFD: black bar; HFD + eplerenone mice, HFD-E: gray bar) were analyzed. Representative photomicrograph of hematoxylin and eosin (HE)-stained sections of eWAT are shown (upper panel). (A) Average size of epididymal white adipocytes (eWAT). Scale bar, 50 μm. Representative photomicrograph of hematoxylin and eosin (HE)-stained sections of the liver (middle panel). Scale bar, 50 μm. Representative photomicrograph of ORO-stained sections of the liver (lower panel). Scale bar, 50 μm. (B) PPARgamma mRNA expression in the eWAT. (C) Triglyceride content in the liver. Data are shown as the mean ± SEM (n = 12). The sizes of eWAT and lipid droplets in the liver were measured with ImageJ. The area of over 200 adipocytes was measured for each tissue sample. Data are presented as the mean ± SEM. Graphs were analyzed using Kruskal-Wallis and Dunn's tests.