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. 2020 Apr 21;11:223. doi: 10.3389/fendo.2020.00223

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Copyright © 2020 Vecchiola, Fuentes, Solar, Lagos, Opazo, Muñoz-Durango, Riedel, Owen, Kalergis and Fardella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of eplerenone on aldosterone plasma levels and RAAS molecule expression in eWAT. Three groups of mice (normal diet-fed mice, ND: white bar; high-fat diet-fed mice, HFD: black bar; HFD + eplerenone mice, HFD-E: gray bar) were analyzed. Plasma aldosterone levels (A) and the aldosterone-renin ratio (B). mRNA expression levels of AT1R, AT2R, CYP11B2, and mineralocorticoid receptor (MR) genes in the eWAT of each group are shown (C). Data are represented as the mean ± SEM. P < 0.05 was considered significant. Graphs (A–C) were analyzed using Kruskal-Wallis and Dunn's tests.