Figure 6.

Upper panel: Effects of eplerenone on the mRNA expression levels of metabolic and inflammatory molecule genes in the eWAT (A–C). Three groups of mice (normal diet-fed mice, ND: white bar; high-fat diet-fed mice, HFD: black bar; HFD + eplerenone mice, HFD-E: gray bar) were analyzed. Data are presented as the mean ± SEM. P < 0.05 was considered significant. Graphs were analyzed using Kruskal-Wallis and Dunn's tests. Lower panels: Eplerenone treatment decreased the percentage of MCHII⁺CD11b^high cells in the SVF. The percentage of MCHII⁺ (a,f), MHCII⁺CD11c⁺ (b,g), MHCII⁺CD11c⁺CD80⁺ (c,h), MHCII⁺CD11c⁺CD80⁺ CD11b^high (d,i), and MHCII⁺CD11c⁺CD80⁺CD11b^low (e,j) was analyzed in the SVF and spleen of ND, HFD, and HFD-E mice. The obtained results showed an increase in MHCII⁺CD11c⁺CD80⁺ CD11b^high in the HFD mice that was reversed by eplerenone treatment. Mean ± SEM. N = 3, ANOVA and Tukey's post-hoc tests. Graph (D) was analyzed via one way ANOVA test.