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. 2020 Apr 21;7:203. doi: 10.3389/fvets.2020.00203

TABLE 2A.

Clinical samples used in this study and results obtained by the dRRT-PCR and the RT-PCR methods used as reference; experimental samples from in vivo experiments performed at INIA-CISA.

Virus Species Animal Id Type of Sample DPI* DUPLEX RRT-PCR WNV-1/WNV-2/USUV RRT-PCRa BAGV RRT-PCRb (Ct)
JE (Ct) NTAYA (Ct) WNV-1 (Ct) WNV-2 (Ct) USUV (Ct)
WNV L1 (GE-1b/B Spain 2007) House sparrow 11 Brain 7 28.44 No Ct 31.25 No Ct No Ct
Kidney 27.37 No Ct 29.15 No Ct No Ct
Spleen 24.01 No Ct 25.68 No Ct No Ct
Heart 26.85 No Ct 28.53 No Ct No Ct
Liver 24.78 No Ct 27.15 No Ct No Ct
WNV L2 (Austria/2008) Red-legged partridge 7 Brain 7 28.41 No Ct No Ct 32.45 No Ct
Kidney 26.43 No Ct No Ct 31.20 No Ct
Spleen 27.6 No Ct No Ct 35.19 No Ct
Heart 24.48 No Ct No Ct 30.89 No Ct
Liver 36.75 No Ct No Ct 39.03 No Ct
Blood 35.84 No Ct No Ct No Ct No Ct
Feather 23.38 No Ct No Ct 30.01 No Ct
BAGV (Spain H/2010) Red-legged partridge 4 Brain 6 No Ct 31.19 29.54
Kidney No Ct 26.17 25.81
Spleen No Ct 24.31 25.69
Heart No Ct 23.47 25.81
Liver No Ct 26.05 26.84
Grey partridge 4 Brain 4 No Ct 35.83 27.11
Kidney No Ct 22.97 21.19
Spleen No Ct 24.27 19.66
Heart No Ct 29.16 20.5
Liver No Ct 26.37 22.45
Blood No Ct 24.95 20.81
Feather No Ct 23.28 19.98
*

DPI: days-post-infection. aDel Amo et al. (34). bBuitrago et al. (35).