TABLE 2B.

Clinical samples used in this study and results obtained by the dRRT-PCR and the RT-PCR methods used as reference; field samples from wild birds collected in Spain.

Virus	Species	Source/ Animal Id	Type of Sample	DUPLEX RRT-PCR		WNV-1/WNV-2/USUV RRT-PCRa			BAGV RRT-PCRb (Ct)
				JE (Ct)	NTAYA (Ct)	WNV-1 (Ct)	WNV-2 (Ct)	USUV (Ct)
WNV L1	Red-legged partridge	LCV/30/11	Brain	28.54	No Ct	31.36	No Ct	No Ct
		LCV/2496/15	Cloacal swab	38.10	No Ct	33.71	No Ct	No Ct
		LCV/3179/13	Oral swab	31.60	No Ct	34.83	No Ct	No Ct
	White stork	LCV/2439/16-1	Feather	36.89	No Ct	No Ct	No Ct	No Ct
	Black vulture	LCV/2442/16-5	Brain	24.34	No Ct	28.17	No Ct	No Ct
		LCV/2442/16-4	Feather	28.28	No Ct	30.75	No Ct	No Ct
	Cinereous vulture	LCV/2442/16-3	Feather	31.66	No Ct	31.49	No Ct	No Ct
	Duck	LCV/2439/16-11	Feather	27.58	No Ct	30.43	No Ct	No Ct
		LCV/2858/16	Feather	34.10	No Ct	39.15	No Ct	No Ct
	Little owl	UEX/10B	Feather	22.34	No Ct	26.36	No Ct	No Ct
			Brain	24.65	No Ct	25.69	No Ct	No Ct
			Liver	24.67	No Ct	27.64	No Ct	No Ct
			Lung	18.79	No Ct	21.54	No Ct	No Ct
			Heart	23.21	No Ct	26.09	No Ct	No Ct
			Spleen	34.38	No Ct	38.32	No Ct	No Ct
		UEX/A	Feather	19.38	No Ct	22.26	No Ct	No Ct
			Brain	27.62	No Ct	29.25	No Ct	No Ct
			Liver	25.26	No Ct	28.48	No Ct	No Ct
			Lung	18.85	No Ct	21.89	No Ct	No Ct
			Heart	19.70	No Ct	22.96	No Ct	No Ct
			Spleen	26.77	No Ct	30.64	No Ct	No Ct
			Kidney	24.21	No Ct	25.51	No Ct	No Ct
USUV	Common blackbird	IREC/A18/177	Oral swab	35.99	No Ct	No Ct	No Ct	39.37
			Cloacal swab	No Ct	No Ct	No Ct	No Ct	No Ct
BAGV	Red-legged partridge	EBD/2010A	Feather	No Ct	24.98				23.26
			Brain	No Ct	28.98				28.62
			Kidney	No Ct	28.86				28.3
			Eye	No Ct	22.72				22.47
			Heart	No Ct	32.93				33.13
			Muscle	No Ct	32				32.08
			Skin	No Ct	29.98				28.34

Only the results of the individuals giving positive result to any JE or Ntaya flavivirus serogroup are shown.

^aDel Amo et al. (34).

^bBuitrago et al. (35).