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. 2020 Apr 21;11:180. doi: 10.3389/fendo.2020.00180

Figure 5.

Figure 5

Overexpression of dilp1 affects body mass and feeding in young adult flies. (A, B) Overexpression of dilp1, dilp2, and dilp6 in fat body (ppl-Gal4) all lead to adult flies (1 week old) with increased body weight in both females and males. Data are presented as means ± S.E.M, n = 24–30 flies for each genotype from three independent replicates. Except for ppl>dilp2, 13 flies were used (*p < 0.05, one-way ANOVA followed with Tukey's test). (C) Body weight of 6- to 7-day female flies is increased for all genotypes compared to 1-day flies and the ppl>dilp1 flies weigh more than controls at both time points. Data are presented as medians ± range, n = 23–27 flies for each genotype from three independent replicates (*p < 0.05, ***p < 0.001, two-way ANOVA followed with Tukey's test). (D) Food intake (CAFE assay) is increased over 4 days (cumulative data shown) in flies overexpressing dilp1 in fat body, but not in neuroendocrine cells (c929-Gal4). Data are presented as means ± S.E.M, n = 15–30 flies for each genotype from three independent replicates (*p < 0.05, two-way ANOVA followed with Tukey's test).