Figure 6.

Effects of mutated dilp genes on adult responses to starvation and desiccation change in early adult life. (A) In newly eclosed female flies, dilp1 mutant flies display reduced survival during starvation (p < 0.001) compared to the other mutants and control. The double mutant is significantly more resistant (p < 0.001). n = 109–147 flies for each genotype from three independent replicates. (B) In 3-day-old virgin female flies, dilp1-dilp2 double mutants are the least starvation resistant (p < 0.001) followed by the dilp1 mutants; n = 129–148 flies for each genotype from three independent replicates. (C) Comparison between newly eclosed and 3-day-old flies exposed to starvation. Both mutants and controls survive longer as recently eclosed flies and mutants perform worse than controls at each time point (p < 0.001). n = 114–144 flies from three independent replicates. (D) When exposed to desiccation, 6- to 7-day-old female double mutants are less resistant than the other genotypes (p < 0.001), n = 132–135 flies from three independent replicates. Data are presented in survival curves and the error bars show S.E.M., as assessed by log-rank (Mantel–Cox) test.