Figure 7.

Overexpression of dilp1 in the fat body affects starvation resistance in adult flies. (A, B) In recently eclosed (A) and 6- to 7-day-old (B) female flies, overexpression of dilp1 (with ppl-Gal4) leads to a decrease in survival during starvation n = 147–201 flies per genotype from three independent replicates [***p < 0.001, as assessed by log-rank (Mantel–Cox) test]. (C,D) Expressing dilp1 in IPCs with a dilp2-Gal4 driver also diminishes starvation survival in recently eclosed flies, n = 92–148 flies from three independent replicates [***p < 0.001, as assessed by log-rank (Mantel–Cox) test], but not in 6- to 7-day flies (n = 122–132 flies from three independent replicates). (E,F) Using c929 to drive dilp1 in recently eclosed and 6- to 7-day-old adult flies altered starvation resistance only in the recently eclosed ones [***p < 0.001 as assessed by log-rank (Mantel–Cox) test, n = 132–135 flies per genotype from three independent replicates].