Figure 8.

Overexpression of dilp1 affects food intake and fecundity. (A) In CAFE assay, the dilp1 overexpressing flies (6- to 7-day-old females) display increased food intake over 4 days (cumulative data shown). Data are presented as means ± S.E.M, n = 23–24 flies from three independent replicates (*p < 0.05, two-way ANOVA followed by Tukey's test). (B) Number of eggs laid in 24 h by 6- to 7-day-old flies. We analyzed 19–29 pairs of flies from 3 replicates (**p < 0.01, one-way ANOVA followed by Tukey's test). (C) The egg to pupal viability is diminished in flies with dilp1 expressed in fat body (ppl-Gal4) and neuroendocrine cells [c929-Gal4, using two different UAS-dilp1 (2 and 3)]. Data are presented as means ± S.E.M; more than 276 eggs from 6 replicates were monitored (*p < 0.05, unpaired Student's t-test). (D) Number of eggs in ovaries of 3-day-old flies is not affected in dilp1 mutants. A total of 25–33 flies from three replicates were analyzed. (E) dilp1 mRNA is upregulated during starvation for 40 h in 10-day-old adult w¹¹¹⁸ flies, compared to 12-day-old flies fed normal food, as monitored by qPCR. No effect was seen on dilp2 and dilp6 levels. Data are presented as means ± S.E.M.; 3 replicates with 10 flies in each replicates were monitored (*p < 0.05, unpaired Student's t-test).