Figure 2.

mTOR inhibitor RAD001 induced anti-tumor effects in vitro. (A) RAD001 efficiently inhibited cell proliferation in a dose dependent manner. WST-1 absorption was tested after 72 h treatment. The experiments were performed in triplicate. *p < 0.05 vs. control. (B) Cells treated with 100 nM RAD001 for 72 h were tested for apoptotic rate. Higher apoptotic rates were observed in the cell samples treated with RAD001. (C) Cells treated with 100 nM RAD001 for 72 h were seeded into 6-well plate for colony formation. Decreased clone numbers were observed in the treated group (n = 6 for each group, *p < 0.05: RAD001 treated group vs. control group).