Figure 3.

RAD001 treatment prolonged the survival time of the CEA424-SV40 TAg transgenic mice. (A) SV40-TAg staining was applied to stomach sections from mice of different age. In vivo experiment started with mice of day-50, when tumors were well-formed. Scale bars: 50 μm. (B) Left: weight curves of the CEA424-SV40 TAg transgenic mice. Blue curve: control group; red curve: RAD001 treated group. Right: mean age of the CEA424-SV40 TAg transgenic mice when they lost 20% of the peak weight (*p < 0.0001: RAD001 treated group vs. control group). (C) Macroscopic picture of the stomachs from control mice and RAD001 treated mice after 48 days of treatment with placebo or RAD001. Mean tumor weight of these two groups was shown in (D) (*p < 0.05: RAD001 treated group vs. control group). (E) An example of the Ki67 staining on stomach sections from control mice and RAD001 treated mice. Scale bars: 50 μm. No significant difference was found for the Ki-67 index in the two groups (F).