Figure 3.

Bintrafusp alfa treatment is associated with increased T-cell infiltration/activation in the TME. Mice harboring HTB-1 subcutaneous tumors were treated with bintrafusp alfa or an IgG1 isotype control as outlined in Figure 2A. Number of IFNγ-producing CD4⁺ (A) and CD8⁺ (B) T cells per milligram tumor weight and frequency of (C) CD8⁺ NKG2D⁺ T cells and (D) IFNγ/GzmB⁺/NKG2D⁺ of total CD8⁺ tumor infiltrating cells at day 21 post-initiation of bintrafusp alfa treatment (*p < 0.05, student's t-test).