Figure 6.

ACPPs reveal cell-type-specific kinetics of gelatinase activity during epileptogenesis. The gelatinase spatiotemporal activation profile was observed with gelatinase-ACPP uptake in CA1 and molecular and granular layers of KA-injected hippocampi at 24 h, 7 days after KA injection or during the chronic phase. Slices were stained with neurons marker NeuN (A–C,J–L), microglial marker Iba1 (D–F,M–O) or astrocytic marker GFAP (G–I,P–R). Merge of the cell type marker (green) and TAMRA ACPP (orange) are shown. n = 3 independent experiments, two or three animals injected per condition for each experiment, three slices per mouse were used. Scale bar 20 μm.