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. 2020 Apr 24;22(6):231–241. doi: 10.1016/j.neo.2020.04.001

Fig. 7.

Fig. 7

PBK promotes neuroblastoma self-renewal and migration. (A–C) Tumorsphere quantitation in control and PBK-depleted neuroblastoma cell lines SKNDZ (A), Kelly (B), and IMR5 (C). Cell lines were infected with control lentiviruses or lentiviruses expressing three independent shRNAs directed against PBK. Western blotting of PBK and Ku80 in cell lines in corresponding cell lines. (D-F) Depicted are representative images from wound migration assays of control and PBK-depleted neuroblastoma cell lines SKNDZ (D), Kelly (E), and IMR5 (F). Cell lines were infected with control lentiviruses or lentiviruses expressing shRNAs directed against PBK. In addition, cells were treated with mitomycin C. Images were acquired at 0 and 24 hours. The relative percentage of wound area occupied by migrated cells 24 hours after scratch was calculated relative to the original wound area using IncuCyte ZOOM analysis software. The percentage of wound closure in control group was normalized to 1, and the fold change of wound closure in experimental groups was calculated. **p < 0.01, ***p < 0.001, ****, p < 0.0001. See also Supplementary Fig. S7.