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. 2020 Apr 21;11:581. doi: 10.3389/fmicb.2020.00581

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Copyright © 2020 Herpell, Schindler, Bejtović, Fragner, Diallo, Bellaire, Kublik, Foesel, Gschwendtner, Kerou, Schloter and Weckwerth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Microscopic analysis of bacteria associated to secretory trichomes on the D. bulbifera leaf acumen. Confocal laser scanning microscopy (CLSM) images of trichomes located on the upper side of the leaf acumen. Trichome autofluorescence could be separated from other channels and can be observed in cyan, chlorophyll autofluorescence in magenta and nucleic acids were stained with SYBR^® < /cps:sup > -Green, here depicted in yellow. Excitation (ex.) and emission (em.) wavelengths are indicated above. (A) Trichomes densely cover the surface of the acumen and microorganisms strongly colocalize with trichomes, as can be observed in panel (B) a magnification of two trichomes in panel (A), as well as in panel (C), showing another pair of trichomes densely colonized by microorganisms. Scale bars represent 20 and 10 μm in panels (A) and (B,C), respectively.