FIGURE 7.

Ecological data concerning Burkholderia sensu lato species within a robust phylogenetic framework. A phylogenetic tree for the genus Burkholderia sensu lato based on the concatenated alignment of 54 ribosomal proteins. Species and strain names represent those used in the Bacterial Isolate Genome Sequence Database (BIGSdb), except for strain Msb3, which is marked in pink and carries the species name proposed in this study. The most recent genus names validly published for specific groups, distinguished through gray and white shading, are shown outside of the first circle. The first circle indicates whether one or more pathogenic strains (plant or animal) were found within a bacterial species. If there was evidence, all strains carrying the same species name were marked yellow. The second circle shows the primary isolation source of every strain included in the analysis, using a color code explained in the legend. Annotations on the tree include small circles on branch tips, showing additional details about the primary isolation source or the isolation source of strains from the same species not included in the analysis. Octagons on nodes display values for ultrafast (UF) bootstrap and approximate likelihood ratio test (aLRT) calculations with the non-parametric SH correction (SH-aLRT) in the spirit of the Shimodaira–Hasegawa (SH) algorithm. Branch length is given as amino-acid substitutions per site. Ralstonia picketti strain 12J was used as an outgroup to set the root of the tree.