. 2020 Apr 21;11:501. doi: 10.3389/fphar.2020.00501

Table 1.

The information of probes in this MLPA assay.

Species	Probe name	Sequence(5′→3′)	Fragment size	Calculated size
B. multicinctus	LPO	GGGTTCCCTAAGGGTTGGATACCAATCATAATCGGAGGGTTTGGCAACT	102	101
B. multicinctus	RPO	GACTTATCCCTTTAATAATCGGGGCCCCTGTCTAGATTGGATCTTGCTGGCAC	102	101
D. acutus	LPO	GGGTTCCCTAAGGGTTGGA T ATCTTTTCTCTCCACTTAGCCGGGGCATCCTCTA	112	110
D. acutus	RPO	TCCTAGGGGCAATTAACTTCATCACTACGTGCATCTCTAGATTGGATCTTGCTGGCAC	112	110
N. atra	LPO	GGGTTCCCTAAGGGTTGGA G TGCCTAAGCATACTAATACGCATAGAACTGACCCAGCCCGGATCACTA	139	137
N. atra	RPO	TTCGGCAGTGACCAGATCTTCAACGTACTTGTAACTGCCCACGCATTTTCTAGATTGGATCTTGCTGGCAC	139	137
S. annularis	LPO	GGGTTCCCTAAGGGTTGGA T ACAGTGTACCCGCCATTGTCTGGAAAC	97	95
S. annularis	RPO	CTAGTACATTCAGGCCCATCGGTGGACTCTAGATTGGATCTTGCTGGCAC	97	95
X. flavipunctatus	LPO	GGGTTCCCTAAGGGTTGGA T TACCCATCATGATCGGTGGTTTCGGGAACTGA	107	105
X. flavipunctatus	RPO	CTAATCCCCCTCATACTAGGAGCCCCCGACATTCTAGATTGGATCTTGCTGGCAC	107	105

Uppercase bold: Primer binding sequence; Uppercase: Hybridizing sequence; Uppercase italics: Stuffer base. All RPOs are 5′-phosphorylated.