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. 2020 Apr 21;11:713. doi: 10.3389/fimmu.2020.00713

Figure 6.

Figure 6

Effect of exogenous IL-15 treatment on phenotypes of natural killer (NK) cells present in cord blood mononuclear cells (CBMC) with comparison to adult peripheral blood (PBMC). (A) Gating strategy used to identify total and subsets of NK cells. Data representing all CBMC samples (n = 30, 15 CBMC donors +/- IL-15) were combined and included in the flow cytometry plots shown. (B) Comparison of NK cell subset frequencies between untreated, and after overnight treatment with 10 ng/mL IL-15 (n = 15 CBMC and n = 15 PBMC samples per condition). Frequencies of total NK cells expressing (C) CD57, (D) CD62L, (E), HLA-DR, and (F) CD69 in cells left untreated or treated overnight with 10 ng/mL IL-15. Amount of intracellular (G) Perforin and (H) Granzyme B in NK cells left untreated or after overnight treatment with 10 ng/mL IL-15, measured by antibody binding capacity (see Methods). In (B–H), box plots represent the interquartile ranges, horizontal lines indicate the medians, and error bars extend to the minimum and maximum observed values.