FIG 2.

Regulatory elements of the sci1 and 4532 promoters. (A and B) Nucleotide sequences of the sci1 (A) and EC042_4532 (B) promoters highlighting overlaps between the transcriptional elements, Fur binding boxes, and Dam methylation motifs. The +1 transcriptional site identified by 5′RACE is indicated in red. GATC Dam methylation sites are indicated in blue. The −10 elements are indicated in green. The underlined sequences indicate Fur binding boxes (italics) and translational start codons. (C) Sequence alignment of the fur1 (sci1 promoter) and fur-32 (EC042_4532 promoter) boxes with the E. coli Fur box consensus sequence. Identical bases are framed in gray. The −10 elements (green) and GATC motifs (blue) are indicated.