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GATC-32 methylation influences Fur binding on P4532. (A) Electrophoretic mobility shift assay of the unmethylated (P4532) or methylated (me-P4532) P4532 fragment with the indicated concentration of purified Fur. (B) Densitometry analysis of Fur binding on the unmethylated or methylated P4532 fragment, represented as free P4532 DNA as a function of Fur concentration.
