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. 2020 Apr 21;11:392. doi: 10.3389/fpls.2020.00392

FIGURE 7.

FIGURE 7

(A) Part of the Plexiglas holder, viewed from above, which was used to pretreat the apical 0–3 mm region of the primary root of line FR697 with the H2O2 scavenger KI. The holder contained a 5-mm thick layer of 1% agarose gel, with or without KI, into which the root tip was inserted. Because the roots elongated during the pretreatment, the kernels were moved away from the gel every 2 h such that only the apical 3 mm of the roots were embedded in the gel. After 6 h, the seedlings were transplanted to well-watered or water-stressed conditions. (B) Elongation rates of control and KI-pretreated (30 or 45 mM) primary roots after transplanting to well-watered (WW) or water-stressed (WS; –1.6 MPa) conditions. The elongation rates are plotted at the midpoint between marking intervals, which were at 0, 16, 24, 40, and 48 h after transplanting. Data are means ± SE of three independent experiments (n = 15–40 seedlings per treatment in each experiment). Different letters denote significant differences between treatments at specific times within each condition (t-test; P < 0.05).