Figure 7.

Tpm3.1 Inhibition Leads to the Loss of Voltage-Gated Sodium Channels Clustering at the AIS

(A) MAP2 and panNa_v immunofluorescence in rat hippocampal neurons incubated overnight at 9–11 DIV in DMSO, LatB, or TR100.

(B) Smoothed panNa_v fluorescence intensity line profiles (gray lines) along each neurite of the corresponding neuron in (A) normalized to the median value (black line).

(C) AIS localization indices for each group (Mann-Whitney U test). Black circles represent mean value. Box borders represent the 25^th and 75^th percentiles, whiskers represent minimum and maximum values less than 1.5x the interquartile range lower or higher than the 25^th or 75^th percentiles, respectively (Tukey style). DMSO 0.2%: n = 17, 3 independent experiments; LatB 5 μM: n = 17, 3 independent experiments; TR100 5 μM: n = 17, 3 independent experiments. ∗ denotes statistical significance. ∗∗∗: p < 0.001. Scale bar: 5 μm.