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. 2020 Apr 21;12:106. doi: 10.3389/fnagi.2020.00106

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Copyright © 2020 Tsuji, Ohshima, Yamamoto, Saito, Hattori, Tanaka, Taguchi, Ihara and Ogawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cerebral blood flow. Cerebral blood flow (CBF) was measured by laser speckle flowmetry at 12 weeks of age. A representative image of surface CBF in 2N and Ts65Dn male mice is shown (A). The region of interest is indicated by the rectangle (solid lines), which was set to be square with bregma and lambda (dashed lines). The average CBF values in the bilateral hemispheres of males (B) and females (C) are shown. In male groups: 2N-Regular (n = 20), 2N-Cilostazol (n = 17), Ts65Dn-Regular (n = 6), Ts65Dn-Cilostazol (n = 14). In female groups: 2N-Regular (n = 8), 2N-Cilostazol (n = 16), Ts65Dn-Regular (n = 12), Ts65Dn-Cilostazol (n = 10). Mean ± SEM. AU, arbitrary unit.