Figure 3.

miR-15b induces apoptotic events in MEG-01 cells. miR-15b mimic or inhibitor (150 nm) was transfected into MEG-01 cells, and miRNA mimic negative control #1 or inhibitor negative control #1 was used as a negative control. Cells were harvested after 12 hours for evaluation of apoptotic events by flow cytometry. Data are mean ± SEM (n=3). (A) Cell apoptosis was assessed with Annexin V and propidium iodide staining. (B) Cell apoptosis was determined with mitochondrial membrane potential (△Ψm) depolarization stained with JC-1 (5 µg/mL). **, P<0.01.