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. 2020 Mar;8(6):327. doi: 10.21037/atm.2020.02.107

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Morusin activates cell apoptosis and perturbs apoptosis-related protein expression. (A) Annexin V PE/7-AAD stained apoptotic cells induced by morusin in RCC cells. (B) Apoptosis ratios were shown in the diagram. (C,D) Apoptosis-related protein expression was detected by western blotting and qPCR in a time and concentration gradient. (^#, *, ^a) P<0.05, (^##, **, ^b) P<0.01, and (^###, ***, ^c) P<0.001 compared to negative control. RCC, renal cell carcinoma; qPCR, real-time quantitative PCR detecting system.