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miR-21 attenuated HK-2 apoptosis and was found to regulate their functions by inhibiting CDK6 expression. HK-2 cells were co-cultured with miR-21 inhibitor or miR-21 inhibitor+CDK6-siRNA or LPS. (A) MTT assay; (B) AO/EB staining; (C) FCM assay; (D) WB assay for Bax and Bcl-2, and quantitative analysis for Bcl-2/Bax ratio. β-Actin was used as a reference gene (n=4). *, P<0.05 vs. LPS group; **, P<0.01 vs. Control group.
