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. 2020 Mar;8(6):378. doi: 10.21037/atm.2020.02.14

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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IGSF3 affects HCC cell proliferation in vitro. (A,B) Western blot analyses of cyclin A, cyclin E, CDK1, and CDK2 levels in HCLLM3 and hepG2 cells. Bar graph shows cyclin A, cyclin D, CDK1, and CDK2 levels in HCLLM3 and hepG2 cells transfected with either shCON or shIGSF3; (C,D) FACS analysis results showing proportion of cells in each phase of the cell cycle. Bar graph shows the results of the number of cells in each phase of the cell cycle. All experiments were conducted three times. Results were indicated as mean ± SD (*, P<0.05; **, P<0.01; ***, P<0.001).