Figure S4.

IGSF3 can promote the NF-κB signaling pathways. (A,B) The relative expression of active NF-kB protein in nuclear of HCC cell lines (HCCLM3 and Huh7) by western blot; (C) Western blot analyses of NF-κB, p-NF-κB, IKBα and p-IKBα expression in the experimental and control group in Huh7 cell line. Bar graph shows the results of quantification of p-IKBα/IKBα and p-NF-κB/NF-κB. All experiments were performed three times. Results were represented as mean ± SD (*, P<0.05; **, P<0.01; ***, P<0.001).