Figure 3.

High expression of SLC1A5 and GPT2 rendered SRCC organoid more sensitive to glutamine deprivation. (A) Western blot showing short hairpin RNA-mediated deletion of SLC1A5 and GPT2 in two organoids; (B) glutamine uptake was examined in Control and GPT2 or SLC1A5 knockdown organoids; (C,D) silence of SLC1A5 and GPT2 significantly inhibited growth of SRCC organoids (light microscope, 200×); (E) control and GPT2 or SLC1A5 knockdown organoids were grown with or without glutamine for 72 h. Cell apoptosis was quantified. *, P<0.05. SRCC, signet-ring cell carcinoma.