Table 1.
ZIKV-80E NPs display EDIII, but not FLE.
| mAb (binding site) | Coating antigena |
|||
|---|---|---|---|---|
| ZSVb | ZIKV-80E | DSV4c | DENV-2-80Ed | |
| ZV-48 (C–C’ loop) | 0.01 | 2.03 | 0.01 | 0.01 |
| ZV-2 (ABDE sheet) | 0.01 | 0.05 | 0.01 | 0.02 |
| ZV-67 (LR) | 0.01 | 1.59 | 0.01 | 0.01 |
| ZKA-64 (EDIII) | 0.01 | 2.50 | 0.01 | 0.01 |
| 24A12 (EDIII)e | 1.40 | 3.30 | 2.59 | 0.94 |
| 4G2 (FLE)f | 0.01 | 0.01 | 0.01 | – |
The coating antigens listed (all are P. pastoris-expressed, purified proteins, assembled into NPs) were probed with mAbs shown in an indirect ELISA format. The assay was performed twice independently. Data shown are average ELISA absorbance values from duplicate assays of one experiment.
Zika subunit vaccine, with ZIKV EDIII displayed on HBV S-based VLPs [48].
Dengue subunit vaccine, with dengue EDIIIs of all four serotypes linked in frame and displayed on HBV S-based VLPs [49].
The N-terminal 80% of DENV-2 E protein self-assembled into NPs [43], similar to the ZIKV-80E described in this manuscript.
This is a flavivirus cross-reactive mAb capable of recognising EDIIIs of ZIKV [48] as well as DENVs 1–3 [53].
This pan-flavivirus-specific mAb was confirmed to recognise DENV-2 virions (in a parallel control assay, data not shown).