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. 2020 Apr 27;3(4):e203652. doi: 10.1001/jamanetworkopen.2020.3652

Figure 2. Patient 29 .

Figure 2.

The patient had a pT3N0 Lauren diffuse subtype adenocarcinoma arising from the gastroesophageal junction with copy number alterations in MET, FGFR2, CCND1, EGFR, CD274, and PDCD1LG2. A, Target 1 images reside at coordinates x = 14 124 μm and y = 23 706 μm and exhibited 3 copies of MET, amplified CCND1, and 3 to 4 copies of FGFR2. Target 2 image resides at coordinates x = 15 145 μm and y = 23 424 μm and exhibited amplified MET. Based on OncoScan data reporting FGFR2 amplification, additional targets were captured after initial analysis. An area of highly amplified FGFR2 was subsequently identified in target 3 (x = 13 505 μm and y = 23 449 μm). Target 4 resides the greatest distance from target 1 at coordinates x = 11 002 μm and y = 27 428 μm and exhibited a normal copy number for CCND1 and FGFR2. B, The tumor area of interest is circled on the hematoxylin-eosin–stained (H&E) slide section with the x, y reference coordinates at the 4 corners of the image displayed in micrometer distances. All fluorescent images were obtained at magnification x60; whole H&E tumor slide section image, magnification x5.