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. 2020 Apr 23;15:2841–2858. doi: 10.2147/IJN.S239608

Figure 7.

Figure 7

The neuroprotective effect of the varying formulations on APP/PS-1 mice. (A) The expression of Bax, Bcl-2 and Cleaved-caspase-3 detected by immunohistochemistry staining in hippocampus and cortex of APP/PS-1 mice, scale bar=50 μm (n=6). (B)-(C) Effect of the varying formulations on brain oxidative stress. The content of MDA (B) and the activity of SOD (C) in brain tissues detected by Kits. Data are presented as mean±SD (n=6). *P<0.05, **P<0.01. (D)-(F) Effect of the varying formulations on neuroinflammation. The levels of TNF-α (D), IL-6 (E) and IL-1β (F) in brain tissues detected by ELISA. Data are presented as mean±SD (n=6). *P<0.05, **P<0.01. (G) Representative images of microglia (Iba-1) in hippocampus and cortex of APP/PS-1 mice by Immunofluorescence staining, scale bar=50 μm, scale bar=10 μm in the insets (n=6). (H) Representative images of Aβ1–42 plaques in hippocampus and cortex by Immunofluorescence staining, scale bar=50 μm, scale bar=10 μm in the insets (n=6). (I) The representative bands of proteins of Aβ1–42. (J)-(K) Semi-quantitative analysis of Aβ1–42 proteins expression by densitometry in the cortex (J) and hippocampus (K) after treatment with the varying formulations. Data are presented as mean±SD (n=6). *P<0.05, **P<0.01. (L) Electron microscopic images from hippocampal CA1 stratum radiatum regions of Control, APP, Ost, Ost-Lip and Tf-Ost-Lip groups. White arrows indicate normal synapses, scale bar=1 μm (n=6).