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. 2020 Apr 14;8(4):e11337. doi: 10.1002/aps3.11337

Figure 1.

Figure 1

Example workflow diagram for cost‐effective target capture sequencing. This workflow is designed to be used by a research group trying targeted sequencing for the first time on a set of 96 samples and represents a 50% reduction in reagent and consumable costs compared with traditional methods (see text). The equipment required for this workflow is a centrifuge capable of 13,000 g, micropipettes (preferably multichannel), −80°C and −20°C freezers, a water bath, a thermocycler, a magnetic bead separator, a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA), and access to a method of hybridization pool quantification (e.g., TapeStation [Agilent Technologies, Santa Clara, California, USA], BioAnalyzer [Agilent Technologies], qPCR).