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Effective enzymatic fragmentation of DNA extractions. Total genomic DNA eluted in 0.1 Tris‐EDTA was run on a 1% agarose gel at 180 V for 60 min and compared with the NEB 1‐kbp DNA ladder (New England Biolabs, Ipswich, Massachusetts, USA). Herbarium samples with a considerable concentration of DNA fragments longer than 500 bp (top) were digested with NEB Fragmentase (New England Biolabs) for 10 min, then run on a new gel (bottom) under the same conditions.
