Figure 3.

Pooling by concentration for hybridization has a minimal effect on target capture efficiency. The DNA concentration of DNA libraries from 95 samples (plus one negative control) spanning 24 species of angiosperms were tested using a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA). Liquid‐phase hybridization with RNA probes (Angiosperms353 version 0.1; Arbor Biosciences, Ann Arbor, Michigan, USA) was conducted in four pools, each containing 24 samples chosen based on their Qubit concentration. Target efficiency is measured as the percentage of sequenced reads mapping to the targeted loci. The four high‐efficiency libraries are from the same species (Nerisyrenia camporum Greene, Brassicaceae), which has a close phylogenetic relationship with target sequences in Angiosperms353.