Table 1.
Comparison of consumable costs between conventional and low‐cost methods in the target capture workflow. See text for full explanation of methods and alternatives and for equipment cost considerations.a
| Hyb‐Seq procedure | Usual technique | Cost‐saving technique | Usual technique price (per sample) | Cost‐saving technique price (per sample) | Estimated fold‐cost savings |
|---|---|---|---|---|---|
| DNA extraction | QIAGEN DNeasy 96 | CTAB | $3.11 | $0.29 | 10.72 |
| Quality control | TapeStation | Qubit + gel | $3.21 | $1.30 | 2.47 |
| Fragmentation | Sonicator | Fragmentase | $6.76 | $1.41 | 4.79 |
| Library prep | NEBNext Ultra II DNA full volume | NEB half volume | $29.20 | $14.60 | 2.00 |
| Purification | AMPure beads | Homebrew beads | $2.26 | $0.08 | 28.25 |
| myBaits | 24‐plex | 24‐plex + dilute myBaits | $2.16 | $0.56 | 3.86 |
| Sequencing | MiSeq 2 × 300 bp (96‐plex) | HiSeq X (384‐plex) | $18.50 | $4.42 | 4.19 |
| Overall | $65.20 | $22.66 | 2.88 |
a
Prices (in U.S. dollars) are approximate and may vary by institution.