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. 2019 Dec 10;101(6):1349–1367. doi: 10.1111/tpj.14596

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© 2019 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Duplication of ABI3 in Xerophyta and loss of the B3 domain.

Maximum‐likelihood phylogenetic tree showing the relationship between AtABI3 and the predicted ABI3 paralogues in Xerophyta humilis, X. viscosa and X. elegans, using full‐length amino acid sequences and 1000 bootstrap replicates. Positions of the conserved PST, B1, B2 and B3 domains are given by shaded segments. The X. elegans ABI3B sequence corresponds to a partial open reading frame (ORF) that is missing the N‐terminal region (dashed line). The conserved stop codon sequences for the Xerophyta‐specific ABI3B, ABI3C and ABI3D genes are shown on the right, with the terminal region of the B2 domain underlined. Protein alignments and phylogenetic analysis performed using Clustal Omega and RAxML.